Journal: The Journal of Clinical Investigation

Article Title: Podocyte histone deacetylase activity regulates murine and human glomerular diseases

doi: 10.1172/JCI124030

Figure Lengend Snippet: (A) Representative glomerular immunofluorescence images of talin-1 (green) and nephrin (red) in doxycycline-induced (Dox-induced) control and Tln1fl/fl Pod-rtTA TetO-Cre mice. Scale bar: 10 μm. (B) Quantification of urine albumin/creatinine ratio in control and Tln1fl/fl Pod-rtTA TetO-Cre mice at 0, 2, and 4 weeks after completion of Dox induction. *P < 0.05 vs. control; n = 5. (C) Plasma creatinine (Cr) levels in control and Tln1fl/fl Pod-rtTA TetO-Cre mice treated with Dox at 0 and 4 weeks after completion of Dox induction. *P < 0.05 vs. control; n = 5. (D) Representative light microscope images (H&E, periodic acid–Schiff [PAS], and trichrome) of Dox-induced control and Tln1fl/fl Pod-rtTA TetO-Cre mouse glomeruli. Arrowheads show mesangial matrix deposition and mesangial cell proliferation. Scale bar: 25 μm. (E) Representative trichrome staining in control and Tln1fl/fl Pod-rtTA TetO-Cre mouse kidneys at representative time points. Arrowheads depict dilated tubules and proteinaceous casts, and arrows display interstitial fibrosis. Scale bar: 50 μm. (F) Representative transmission electron micrograph (TEM) in control and Tln1fl/fl Pod-rtTA TetO-Cre mouse foot processes after Dox induction. Arrowheads depict podocyte foot process effacement. Scale bar: 1 μm. (G) Quantification of glomerulosclerosis in D. *P < 0.05 vs. control. (H) Quantification of interstitial fibrosis in E. *P < 0.05 vs. control. (I) Quantification of foot processes in F. *P < 0.05 vs. control. (J) Quantification of WT1-positive number per glomerulus in control and Tln1fl/fl Pod-rtTA TetO-Cre mice treated with Dox at 0 and 4 weeks after completion of Dox induction. *P < 0.05 vs. control; n = 3. (B, C, and G–J) Statistically analyzed by 1-way ANOVA with Dunnett’s correction.

Article Snippet: Mouse anti–Wilms tumor 1 (anti-WT1) (EMD Millipore Corp., catalog 05-753); rabbit anti-WT1 (Santa Cruz Biotechnology, catalog sc-192); mouse anti-HDAC1 (catalog 5356), mouse anti-HDAC2 (catalog 5113), rabbit anti–serum response factor (anti-SRF) (catalog 5147), rabbit anti–CRE-binding protein (anti-CREB) (catalog 9197), rabbit anti–phosphorylated CREB (Ser133) (catalog 9198), rabbit anti-EGR1 (catalog 4154), and rabbit anti-GAPDH (catalog 5174) (Cell Signaling Technology); mouse anti–talin-1 (Bio-Rad, catalog MCA4770); guinea pig anti-nephrin (Progen, catalog GP-N2); rabbit anti-EGR1 (Protein Tech, catalog 55117-1-AP); Alexa Fluor 594 phalloidin (catalog A12381), Alexa Fluor 488 goat anti-mouse IgG antibody (catalog A-11001), Alexa Fluor 594 goat anti–guinea pig IgG antibody (catalog A-11076), and Alexa Fluor 594 goat anti-rabbit IgG antibody (catalog A-11037) (Invitrogen) were purchased commercially. eGFP-EGR1 plasmid was purchased from Addgene.

Techniques: Immunofluorescence, Light Microscopy, Staining, Transmission Assay

Journal: Journal of Cancer

Article Title: Inward Tension of Talin and Integrin-related Osmotic Pressure are involved Synergetically in the Invasion and Metastasis of Non-small Cell Lung Cancer

doi: 10.7150/jca.45494

Figure Lengend Snippet: Talin-1 rather than talin-2 is associated with malignancy in NSCLC ( A ) Kaplan-Meier plots of overall survival of patients with squamous carcinoma and adenocarcinoma, stratified by talin-1 and talin-2 expression. ( B ) Western blotting of talin protein levels in fresh NSCLC tissue ( C ) and adjacent normal tissue (P). (C) Normalized quantitative analysis of the wound closure rate in A549 cells transfected with control siRNA or talin-1 siRNA. ( D ) Quantitative analysis of the migration and invasion rates. **P <0.01 compared with the control group, ***P <0.001 compared with the control group.

Article Snippet: Antibody against talin (A02859-1, Western blotting 1:500) was purchased from Boster Bio (Pleasanton, CA, USA).

Techniques: Expressing, Western Blot, Transfection, Control, Migration